Overall information
List of available PhD theses - Department of Analytical Chemistry:
Isolation of biologically active compounds from Sutherlandia and identification of their molecular structure
Annotation
Sutherlandia plants have been identified as being useful in the treatment of various diseases. The aim of this work is to isolate new biologically active substances, to identify their molecular structure using high-resolution NMR and MS, and to describe their biological activity in cooperation with screening centres.
Preparation of solid surfaces with a molecular receptor covalently anchored, and studies of their usability in sensors construction
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The aim of this work is to modify the chemical structure of molecular receptors functional in solution so that they can be covalently attached to a solid surface or prepare a polymer. Study the functionality and usability of such materials for sensor construction.
Spectroscopic Studies of Protein Fibrils
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Many biological processes involve aggregation of proteins into insoluble aggregates, "fibrils". Their properties are studied, for example, to prevent neurodegenerative diseases limiting human live time. We use optical spectroscopic, computational and other methods to understand basic principles of fibril behavior and structure. Particular aim of the thesis is to develop measurement and interpretation procedures for the modern spectroscopy of Raman optical activity, the topic involves spectroscopic experiments as well as computational modeling.
Study of porphyrin derivatives of Tröger and spiro-Tröger bases enabling recognition of cancer cells and photodynamic therapy
Annotation
A major problem in the treatment of cancer is the recognition of healthy cells from cancer and the selective removal or killing of cancer cells. Several substances that are used for visual diagnosis and photodynamic treatment are from the group of porhyrin and chlorine derivatives. The aim of this work is to prepare porphyrin, chlorine and bacteriochlorin derivatives of Tröger and spiro-Tröger bases and to study their usefulness for selective recognition of cancer cells and subsequent photodynamic therapy.
Surface enhanced fluorescence as a tool for nanotransporter localization in a cell
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The project will deal with the development of fluorescence analysis methods for reliable detection of the localization of drug nanocarrires in a living cell. Although fluorescence methods show favorable detection limits in many cases, many organic fluorescent substances exhibit toxicity even at very low concentrations. Current methods of fluorescence microscopy make it possible to monitor the localization of practically individual nanoparticles that have penetrated the cell membrane into the cell. Surface-enhanced fluorescence that occurs near metallic nanoparticles may allow the detection of fluorescent substances at such low concentrations that allow the application of completely non-toxic (low) doses of the preparations to study the chemical behavior of living cells in the presence of these transporters.